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The preparation of the CaM:SEF2-1mp NMR samples has been described by us earlier (11)
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These enzymes catalyze the hydrolysis of a minor component in membranes, PI(4,5)P^sub 2^, to generate two second messengers which lead to a rise in intracellular calcium and activation of protein kinase C (see Rebecchi and Pentyala, 2000; Rhee, 2001)
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